Coding

Part:BBa_M41004:Design

Designed by: Hao Xing   Group: MIT BE 20.20-S13   (2013-05-12)


AlkS coding sequence in Pseudomonas putida


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1731
    Illegal SpeI site found at 2100
    Illegal PstI site found at 221
    Illegal PstI site found at 1360
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2100
    Illegal PstI site found at 221
    Illegal PstI site found at 1360
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1973
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1731
    Illegal SpeI site found at 2100
    Illegal PstI site found at 221
    Illegal PstI site found at 1360
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1731
    Illegal SpeI site found at 2100
    Illegal PstI site found at 221
    Illegal PstI site found at 1360
    Illegal NgoMIV site found at 726
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 888
    Illegal BsaI.rc site found at 359
    Illegal BsaI.rc site found at 661


Design Notes

We cannot separate pAlkS1, pAlkS2 and AlkS gene because of self-regulatory system.


Source

Canosa, I., et al. "A Positive Feedback Mechanism Controls Expression of AlkS, the Transcriptional Regulator of the Pseudomonas Oleovorans Alkane Degradation Pathway." Molecular microbiology 35.4 (2000): 791-9. Van Beilen, JB, et al. "Analysis of Pseudomonas Putida Alkane-Degradation Gene Clusters and Flanking Insertion Sequences: Evolution and Regulation of the Alk Genes." Microbiology 147.6 (2001): 1621-30.


References